Review

formaldehyde (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher formaldehyde
Formaldehyde, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formaldehyde/Formaldehyde%2C+4%25+in+PBS/pm42353371-82-14-29
Average 99 stars, based on 1 article reviews

formaldehyde - by Bioz Stars, 2026-10

99/100 stars

Images

Incubation:

Article Title: Scalable hypothalamic neuron differentiation from human pluripotent stem cells suitable for modeling metabolic disorders.
Article Snippet: Immunocytochemistry and high content imaging analysis hPSC-derived hypothalamic neurons at different time points were cultured as described above on glass-bottom multiwell plates (Cellvis, P24-1.5H-N). .. The cultures were fixed with 4% formaldehyde (Thermo-Fisher, 28908) in PBS for 15 min. Then the cultures were incubated with PBS +4% donkey serum +0.1% Triton X- for 1 h at room temperature, followed by incubation with primary antibodies overnight at 4 ◦ C. Secondary antibodies were incubated at room temperature for 1 h. Refer to Table S1 for complete list of primary and secondary antibodies. .. The cultures were stained with 4 μM Hoechst 33342 (VWR, PI62249) in PBS for 10 min before imaging on the Opera Phenix high-content microscope (Revvity) or Zeiss LSM 710 confocal microscope.

Article Title: An N-terminal amphipathic helix governs activity and conformational dynamics of Nramp metal transporters.
Article Snippet: .. Cells were then fixed using 4% formaldehyde at room temperature for 15 min. After washing three times with DPBS buffer, cells were permeabilized with 0.1% Tween-20 and blocked by 2% BSA in DPBS buffer at room temperature for 1 h. Cells were then incubated with an anti-FLAG antibody at 4 μg/ml and room temperature for 1 h. After washing three times with DPBS, cells were incubated with Alexa Fluor 568-conjugated anti-mouse IgG (Invitrogen, Cat# A-11004) diluted 1:400 in DPBS at room temperature for 2 h. Cells were then washed three times with DPBS, and coverslips were mounted onto slides using Fluoroshield mounting medium containing DAPI. .. Images were taken with a 40 x objective using Nikon C2 confocal microscope.

Fluorescence:

Article Title: Precursor-dependent optical and structural properties of eleven NIR-emissive graphene quantum dots for bioimaging applications
Article Snippet: .. To prepare the slides for NIR fluorescence imaging, the cells were fixed with 4% formaldehyde (Thermo Scientific, 28 908) and mounted with fluoromount-GTM mounting medium (Invitrogen, 00–4958-02). ..

Imaging:

Staining:

Article Title: Cell size-dependent mRNA transcription drives proteome remodeling.
Article Snippet: Cells were induced with doxycycline for 4 or 5 days then seeded onto 35mm imaging dishes (MatTek P35G-1.5-20-C). .. After 2 and 3 days of growth, cells were fixed with 4% formaldehyde (Thermo Scientific; 28906), and after washing twice with PBS, stained with Hoechst33342 (Thermo Scientific; 62249) at 20 μM. ..